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您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 在線活細胞濃度在昆蟲桿狀病毒表達系統(tǒng)sf9中的應(yīng)用
在線活細胞濃度在昆蟲桿狀病毒表達系統(tǒng)sf9中的應(yīng)用
  • 發(fā)布日期:2014-09-19      瀏覽次數(shù):4983
    • Animal cells Sf9動物細胞sf9

      活細胞濃度分析儀  訂購:    索取詳細資料:

      Sf9 Insect cell growth and infection process by a baculovirus (BEVS)
      sf9昆蟲細胞的生長和感染的病毒進程(BEVS
      The FOGALE BIOMASS SYSTEM signal provides    FOGALE BIOMASS 活細胞濃度分析儀提供實時數(shù)據(jù)信號
      direct real time informations on: 直接的實時信息

      >Cell growth monitoring: 細胞生長監(jiān)測:
      The permittivity signal is correlated to viable cell count during the growth phase.

      在細胞的生長階段電容信號與活細胞數(shù)有正相關(guān)性。
      >Time of infection: 感染時刻
      Cells are infected while they are in logarithm growth phase, the time of infection that is of great importance for productivity is accuretly determined.

      細胞受到感染的同時處于對數(shù)生長期,確定準確的感染時間對于生產(chǎn)來說至關(guān)重要!
      >Success of baculovirus infection: 桿狀病毒感染的成功
      The plateau region in the permittivity profile (at 12 to 20h p.i.) has been related as an indicator of infection synchronicity (Zeiser et al, 2000)
      在介電容值的波峰區(qū)(在1220hπ)與細胞感染的同步(Zeiser et al, 2000
      >Harvest time: 產(chǎn)出
      It is of crucial importance for the yield and quality of the recombined protein.

      這對重組蛋白的產(chǎn)量和質(zhì)量來說至關(guān)重要。
      The cell lysis monitoring helps in determining the optimal harvest time during the release phase.

      細胞凋亡裂解的實時監(jiān)測有助于釋放階段確定*采收期

      Permittivity, biovolume in a batch culture of insect cell infected by a baculovirus.

      電容,生物量在單次感染病毒的昆蟲細胞

      Permittivity, viable cell count (trypan blue exclusion method), cell diameter (CASY counter)

      電容,活細胞數(shù)(臺盼藍不相容試驗),細胞直徑(CASY計數(shù)器)